Western blot shows lysates of human small intestine tissue. PVDF Membrane was probed with 0.5 µg/mL of Human AG‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6307) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for AG‑3 at approximately 19kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
AG‑3 was detected in immersion fixed paraffin-embedded sections of human breast using Sheep Anti-Human AG‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6307) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.