Western Blot: CIZ1 Antibody [NBP2-15906] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: CIZ1 Antibody [NBP2-15906] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: CIZ1 Antibody [NBP2-15906] - Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using antibody at 1:500 dilution.
Immunoprecipitation: CIZ1 Antibody [NBP2-15906] - CIZ1 antibody immunoprecipitates CIZ1 protein in IP experiments. IP Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2. 5 ug of preimmune rabbit IgG C. Immunoprecipitation of CIZ1 protein by 2. 5 ug of CIZ1 antibody 7. 5% SDS-PAGE The immunoprecipitated CIZ1 protein was detected by CIZ1 antibody diluted at 1:1000. Anti-rabbit IgG was used as a secondary reagent.