Western Blot: StAR Antibody (5F9) [H00006770-M01] - Analysis of STAR expression in transfected 293T cell line by STAR monoclonal antibody (M01), clone 5F9.Lane 1: STAR transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.
Western Blot: StAR Antibody (5F9) [H00006770-M01] - Analysis of STAR over-expressed 293 cell line, cotransfected with STAR Validated Chimera RNAi ( Cat # H00006770-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with STAR monoclonal antibody (M01), clone 5F9 (Cat # H00006770-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: StAR Antibody (5F9) [H00006770-M01] - Detection limit for recombinant GST tagged STAR is approximately 0.03ng/ml as a capture antibody.