Western Blot: SLC31A1/CTR1 Antibody (5E6.1B4) [NBP2-36573] - Western blot analysis of SLC31A1/CTR1 in MCF7 and HeLa at 2 ug/ml.
Immunocytochemistry/Immunofluorescence: SLC31A1/CTR1 Antibody (5E6.1B4) [NBP2-36573] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti SLC31A1/CTR1 (5E6.1B4) NBP2-36573 at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) [NBP2-36573] - IHC-P analysis of a formalin fixed and paraffin embedded tissue section of human small bowel malignant stromal tumor (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration. The cancer cells developed moderate to strong positivity for CTR1/SLC31A1 protein.
Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) [NBP2-36573] - IHC-P analysis of a formalin fixed and paraffin embedded tissue section of human urinary bladder transitional cell carcinoma (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration.
Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) [NBP2-36573] - IHC-P analysis of a formalin fixed and paraffin embedded tissue section of normal breast tissue from a human breast cancer patient (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration. The antibody generated a very strong cytoplasmic staining in the myo-epithelial cells in the tested section.
Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) [NBP2-36573] - IHC-P analysis of a formalin fixed and paraffin embedded tissue section of normal skin from a human breast cancer patient (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration. The antibody developed a strong cytoplasmic staining in the outer layer of stratum spinosum and the cells of stratum granulosum/ granular layer of skin epidermis. The endothelial cells in the dermis of the section also showed positivity for SLC31A1/CTR1.