Western blot shows lysates of CHP‑100 human neuroblastoma cell line, SH‑SY5Y human neuroblastoma cell line, and Neuro‑2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PTP gamma /PTPRG Monoclonal Antibody (Catalog # MAB4608) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTP gamma /PTPRG at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.