Western blot shows lysates of human erythrocytes and human heart tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human ICAM‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7179) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ICAM‑4 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for ICAM‑4 at approximately 48 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human ICAM‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7179) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Human red blood cells (erythrocytes) were stained with Sheep Anti-Human ICAM‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7179, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).