Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hepa 1‑6 mouse hepatoma cell line, IMR‑32 human neuroblastoma cell line, Neuro‑2A mouse neuroblastoma cell line, and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Inorganic Pyrophosphatase/PPA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6557) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Inorganic Pyrophosphatase/PPA1 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.