Western blot shows lysates of human brain stem tissue, human brain (hypothalamus) tissue, human brain (cerebellum) tissue, mouse brain (cerebellum) tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Endophilin A1/SH3GL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8006) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Endophilin A1/SH3GL2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.