Recombinant Equine IL‑1 beta /IL‑1F2 (Catalog # 3340‑EL) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Equine IL‑1 beta /IL‑1F2 (100 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti‑Equine IL‑1 beta /IL‑1F2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3340). The ND50 is typically 0.05‑0.25 µg/mL in the presence of concanavalin A (1.25 µg/mL).
IL‑1 beta /IL‑1F2 was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Equine IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3340) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.