Carbonic Anhydrase IV was detected in perfusion fixed frozen sections of mouse small intestine using Mouse Carbonic Anhydrase IV Monoclonal Antibody (Catalog # MAB2414) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to nuclei, cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Carbonic Anhydrase IV was detected in perfusion fixed frozen sections of mouse kidney using Mouse Carbonic Anhydrase IV Monoclonal Antibody (Catalog # MAB2414) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.