Western blot shows lysates of LNCaP human prostate cancer cell line, JAR human choriocarcinoma cell line, NTera‑2 human testicular embryonic carcinoma cell line, Neuro‑2A mouse neuroblastoma cell line, and human brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PGPEP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6278) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PGPEP‑1 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.