Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and DA3 mouse myeloma cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse EBF‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5166) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EBF‑3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.