Western Blot: MYBPHL Antibody [NBP2-30018] - Western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the MYBPHL antibody detected the MYBPHL protein (arrow).
Immunocytochemistry/Immunofluorescence: MYBPHL Antibody [NBP2-30018] - Confocal immunofluorescent analysis of (N-term)(NBP2-30018) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Immunohistochemistry-Paraffin: MYBPHL Antibody [NBP2-30018] - Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Flow Cytometry: MYBPHL Antibody [NBP2-30018] - Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.