Western Blot: WDR27 Antibody [NBP2-30017] - Western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the WDR27 antibody detected the WDR27 protein (arrow).
Immunocytochemistry/Immunofluorescence: WDR27 Antibody [NBP2-30017] - Confocal immunofluorescent analysis of (N-term)(NBP2-30017) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Immunohistochemistry-Paraffin: WDR27 Antibody [NBP2-30017] - Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Flow Cytometry: WDR27 Antibody [NBP2-30017] - Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.