Western blot shows lysates of Sf 21 insect cells before (lane 1) and 48 hours post infection with human NAIP (lane 2). PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human NAIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF829) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for truncated NAIP was detected at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.