Smoothelin B was detected in formalin fixed paraffin-embedded sections of human colon using Mouse Anti-Human Smoothelin B Monoclonal Antibody (Catalog # MAB8278) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells in vasculature. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
HASMC human aortic smooth muscle cells was stained with Mouse Anti-Human Smoothelin B Monoclonal Antibody (Catalog # MAB8278, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Intracellular Molecules.