
Western blot shows lysates of human brain (cerebellum) tissue, human brain (cortex) tissue, human brain (hippocampus) tissue, human brain (hypothalamus) tissue, and mouse brain (cerebellum) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Cerebellin-2 Monoclonal Antibody (Catalog # MAB7044) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cerebellin-2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.