Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line and MDA‑MB‑468 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Wnt‑10b Monoclonal Antibody (Catalog # MAB7196) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Wnt‑10b at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysate of SW480 human colorectal adenocarcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Wnt‑10b at approximately 58 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Wnt‑10b Monoclonal Antibody (Catalog # MAB7196). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.