Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line and IMR‑32 human neuroblastoma cell line and cytoplasmic extracts of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human KLF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7016) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for KLF10 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.