
Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse Carbohydrate Sulfotransferase 4/CHST4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5547) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carbohydrate Sulfotransferase 4/CHST4 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX.