Western blot shows lysates of MCF‑7 human breast cancer cell line. Gels were loaded with 25 µg of cytoplasmic (Cyto) extracts. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human S100A13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4327) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100A13 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.