Western blot shows lysates of NK‑92 human natural killer lymphoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Cytohesin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4807) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Cytohesin‑1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.