![Western Blot: Bax inhibitor 1 Antibody (20F248) [NBP2-24949] - Analysis of Bi-1 in A) A549 and B) human lung lysate using this antibody at 1:1000.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/15915_Bax%2520inhibitor%25201%2520Antibody%2520(20F248)-Western%2520Blot-NBP2-24949-img0001.jpg)
Western Blot: Bax inhibitor 1 Antibody (20F248) [NBP2-24949] - Analysis of Bi-1 in A) A549 and B) human lung lysate using this antibody at 1:1000.
![Immunocytochemistry/Immunofluorescence: Bax inhibitor 1 Antibody (20F248) [NBP2-24949] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti Bax inhibitor 1 (20F248), NBP2-24949, at a 1:200 dilution overnight at 4C and detected with an anti-mouse IgM Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/15916_Bax-inhibitor-1-Antibody-(20F248)-Immunocytochemistry-Immunofluorescence-NBP2-24949-img0002.jpg)
Immunocytochemistry/Immunofluorescence: Bax inhibitor 1 Antibody (20F248) [NBP2-24949] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti Bax inhibitor 1 (20F248), NBP2-24949, at a 1:200 dilution overnight at 4C and detected with an anti-mouse IgM Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
![Flow (Intracellular): Bax inhibitor 1 Antibody (20F248) [NBP2-24949] - An intracellular stain was performed on THP-1 cells with Bax Inhibitor 1 (20F248) NBP2-24949 (blue) and a matched isotype control NBP2-27284 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/15917_Bax-inhibitor-1-Antibody-(20F248)-Flow-(Intracellular)-NBP2-24949-img0003.jpg)
Flow (Intracellular): Bax inhibitor 1 Antibody (20F248) [NBP2-24949] - An intracellular stain was performed on THP-1 cells with Bax Inhibitor 1 (20F248) NBP2-24949 (blue) and a matched isotype control NBP2-27284 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.