Western blot shows lysates of SW13 human adrenal cortex adenocarcinoma cell line and IMR‑32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pyruvate Dehydrogenase Phosphatase/PDP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7260) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pyruvate Dehydrogenase Phosphatase/PDP1 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.