Western Blot: Fast skeletal myosin light chain 1 Antibody (2D9) [H00004632-M01] - Western blot analysis of MYL1 over-expressed 293 cell line, cotransfected with MYL1 Validated Chimera RNAi or non-transfected control. Blot probed with H00004632-M01. GAPDH (36.1 kDa) used as loading control.
Immunohistochemistry-Paraffin: Fast skeletal myosin light chain 1 Antibody (2D9) [H00004632-M01] - Immunoperoxidase of monoclonal antibody to MYL1 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]
Western Blot: Fast skeletal myosin light chain 1 Antibody (2D9) [H00004632-M01] - Analysis of MYL1 expression in transfected 293T cell line by MYL1 monoclonal antibody (M01), clone 2D9. Lane 1: MYL1 transfected lysate (21.1 KDa). Lane 2: Non-transfected lysate.
ELISA: Fast skeletal myosin light chain 1 Antibody (2D9) [H00004632-M01] - Detection limit for recombinant GST tagged MYL1 is approximately 3ng/ml as a capture antibody.