Western Blot: ZNF295 Antibody [NB100-74655] - Detection of Human ZNF295 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZNF295 antibody NB100-74655 used for IP at 3 mcg/mg lysate. ZNF295 was also immunoprecipitated by rabbit anti-ZNF295 antibody NB100-74656, which recognizes a downstream epitope.
Immunocytochemistry/Immunofluorescence: ZNF295 Antibody [NB100-74655] - Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100
Immunohistochemistry: ZNF295 Antibody [NB100-74655] - Sample: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZNF295 used at a dilution of 1:1,000 (1ug/ml) and 1:400 (2.5ug/ml).