Western Blot: CoREST3/RCOR3 Antibody [NB100-60637] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NB100-60637 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RCOR3 was also immunoprecipitated by rabbit anti-RCOR3 antibody NB100-60636, which recognizes an upstream epitope.
Immunohistochemistry: CoREST3/RCOR3 Antibody [NB100-60637] - Sample: FFPE sections of human testicular seminoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti- RCOR3 used at a dilution of 1:200 (1ug/ml) and 1:80 (2.5 ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy