Western Blot: EML4 Antibody [NBP1-05973] - Detection of Human EML4 by Western Blot. Samples: Whole cell lysate (50, 15 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EML4 antibody NBP1-05973 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-05973] - Human Lin Plastica stomach cancer. Antibody used at a dilution of1:200.
Western Blot: EML4 Antibody [NBP1-05973] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-05973 used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. EML4 was also immunoprecipitated by NBP1-05974, which recognizes a downstream epitope.
Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-05973] - FFPE section of human lung adenocarcinoma. Affinity purified rabbit anti-EML4 used at a dilution of 1:250.