Western Blot: Anillin Antibody [NB100-68216] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-68216 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Anillin was also immunoprecipitated by NB100-68215, which recognizes an upstream epitope.
Immunohistochemistry: Anillin Antibody [NB100-68216] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Anillin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry-Paraffin: Anillin Antibody [NB100-68216] - FFPE section of human colon carcinoma. Affinity purified rabbit anti-Anillin used at a dilution of 1:250.