Western Blot: PHF6 Antibody [NB100-68260] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PHF6 antibody NB100-68260 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68261 and NB100-68262, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).