![Western Blot: PDHX Antibody [NBP2-30053] - PDX1 (arrow) using PDX1 Antibody (T11) (NBP2-30053). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (Lane 2) (Origene Technologies).](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/70_PDHX-Antibody-Western-Blot-NBP2-30053-img0004.jpg)
Western Blot: PDHX Antibody [NBP2-30053] - PDX1 (arrow) using PDX1 Antibody (T11) (NBP2-30053). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (Lane 2) (Origene Technologies).
![Immunocytochemistry/Immunofluorescence: PDHX Antibody [NBP2-30053] - SY5Y cells stained with PDX1 (T11) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with NBP2-30053 PDX1 (T11) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the PDX1 immunosignal to the nucleus, supported by Human Protein Atlas Data (//www.proteinatlas.org/ENSG00000110435).](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/71_PDHX-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30053-img0002.jpg)
Immunocytochemistry/Immunofluorescence: PDHX Antibody [NBP2-30053] - SY5Y cells stained with PDX1 (T11) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with NBP2-30053 PDX1 (T11) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the PDX1 immunosignal to the nucleus, supported by Human Protein Atlas Data (//www.proteinatlas.org/ENSG00000110435).
![Immunohistochemistry-Paraffin: PDHX Antibody [NBP2-30053] - Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Phospho-PDX1-T11.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/72_PDHX-Antibody-Immunohistochemistry-Paraffin-NBP2-30053-img0005.jpg)
Immunohistochemistry-Paraffin: PDHX Antibody [NBP2-30053] - Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Phospho-PDX1-T11.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
![Western Blot: PDHX Antibody [NBP2-30053] - NCI-H460 cell line lysates (35ug/lane).PDX1-T11(arrow) was detected using the purified Pab.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/73_PDHX-Antibody-Western-Blot-NBP2-30053-img0003.jpg)
Western Blot: PDHX Antibody [NBP2-30053] - NCI-H460 cell line lysates (35ug/lane).PDX1-T11(arrow) was detected using the purified Pab.