Western Blot: ANKH Antibody [NBP2-29950] - Anti-ANKH Antibody at 1:1000 dilution + U-2OS whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry-Paraffin: ANKH Antibody [NBP2-29950] - IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Flow Cytometry: ANKH Antibody [NBP2-29950] - Flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot: ANKH Antibody [NBP2-29950] - Western blot analysis of (C-term) (NBP2-29950) in mouse cerebellum tissue lysates (35ug/lane). ANKH (arrow) was detected using the purified Pab.