Western blot shows lysates of JEG‑3 human epithelial choriocarcinoma cell line and HCT‑116 human colorectal carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FAM3C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3084) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FAM3C at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.