Recombinant Human PRELP (Catalog # 6447-PR) inhibits TRANCE-induced osteoclast differentiation in the RAW 264.7 mouse monocyte/macrophage cell line in a dose-dependent manner (orange line), as measured by the Tartrate-resistant acid phosphatase activity assay. Under these conditions, TRANCE-induced osteoclast differentiation inhibited by Recombinant Human PRELP (5 μg/ml) is neutralized (green line) by increasing concentrations of Mouse Anti-Human PRELP Monoclonal Antibody (Catalog # MAB64471) in the presence of 5 ng/mL Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TEC) and 20 ng/mL Recombinant Mouse M‑CSF (Catalog # 416-ML). The ND50 is typically 0.25‑1.25 μg/mL.