Western blot shows lysates of mouse spleen tissue, mouse colon tissue, and mouse splenocytes. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse CD38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4947) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD38 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for CD38 at approximately 64 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse CD38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4947) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.