Western blot analysis of extracts from MCF7 cells, treated with CIP or TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (200 nM, 10 min), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb #4370. Protein A (HRP Conjugate) was used for detection.
Western blot analysis of extracts from NIH/3T3 cells using Akt (pan) (C67E7) Rabbit mAb #4691. Protein A (HRP Conjugate) was used for detection.