Western Blot: CRLF1 Antibody (4F4) [H00009244-M01] - CRLF1 monoclonal antibody (M01), clone 4F4 Analysis of CRLF1 expression in HeLa.
Western Blot: CRLF1 Antibody (4F4) [H00009244-M01] - Analysis of CRLF1 over-expressed 293 cell line, cotransfected with CRLF1 Validated Chimera RNAi ( Cat # H00009244-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CRLF1 monoclonal antibody (M01), clone 4F4 (Cat # H00009244-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: CRLF1 Antibody (4F4) [H00009244-M01] - Analysis of CRLF1 expression in transfected 293T cell line by CRLF1 monoclonal antibody (M01), clone 4F4.Lane 1: CRLF1 transfected lysate(46.3 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: CRLF1 Antibody (4F4) [H00009244-M01] - Analysis of CRLF1 transfected lysate using anti-CRLF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CRLF1 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: CRLF1 Antibody (4F4) [H00009244-M01] - Detection limit for recombinant GST tagged CRLF1 is approximately 0.03ng/ml as a capture antibody.