Western Blot: SCC112 Antibody [NB100-426] - Samples : Nuclear extract (5 and 20 ug for WB, 1 mg for IP) from HeLa cells. Antibodies : Affinity purified rabbit anti-SCC-112 antibody used at 0.1 ug/ml for WB and at 2 ug/mg extract for IP. Immunoprecipitated SCC-112 was detected with affinity purified rabbit anti-SCC-112 antibody used at 0.06 ug/ml. Detection : Chemiluminescence with an exposure time of less than 5 minutes.
Western Blot: SCC112 Antibody [NB100-426] - Detection of human SCC-112 in Western Blot and immunoprecipitation, using NB100-426. Samples: HeLa nuclear extracts. Exposure time of less than 5 minutes.
Western Blot: SCC112 Antibody [NB100-426] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SCC-112 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Proximity Ligation Assay: SCC112 Antibody [NB100-426] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SA1 (NB100-298) and rabbit anti-human SCC112 (NB100-426). Image merged from DAPI (2ms) and Texas Red (200ms) exposures. 40X magnification.
Proximity Ligation Assay: SCC112 Antibody [NB100-426] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMC1 (NBP2-14858) and rabbit anti-human SCC-112 (NB100-426). Image merged from DAPI (2ms) and Texas Red (200ms) exposures. 40X magnification.