Western Blot: SRP68 Antibody [NBP2-22307] - Whole cell lysate (50 ug) from TCMK-1, Renca, and NIH3T3 cells. NBP2-22307 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: SRP68 Antibody [NBP2-22307] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and Jurkat (50ug) cells. NBP2-22307 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: SRP68 Antibody [NBP2-22307] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. NBP2-22307 used for IP at 6 ug/mg lysate. SRP68 was also immunoprecipitated by rabbit anti-SRP68 antibody. For blotting immunoprecipitated SRP68, NBP2-22307 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.