Western Blot: POLR3D Antibody [NBP1-28737] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-POLR3D antibody NBP1-28737 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. POLR3D was also immunoprecipitated by rabbit anti-POLR3D antibody NBP1-28726, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).
Immunohistochemistry: POLR3D Antibody [NBP1-28737] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- POLR3D used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB