Immunoprecipitation: PI4KB/PI4KIII beta Antibody [NBP2-12814] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PI4KB antibody NBP2-12814 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. PI4KB was also immunoprecipitated by rabbit anti-PI4KB antibody NBP2-12815, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).