![Immunocytochemistry/Immunofluorescence: CACNA2D2 Antibody [NBP2-30044] - Fluorescent image of U251 cell stained with CACNA2D2 Antibody (Center)(NBP2-30044).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CACNA2D2 primary antibody (1:25, 1 h at 37). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37).CACNA2D2 immunoreactivity is localized to Cytoplasm significantly.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/4116_CACNA2D2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30044-img0001.jpg)
Immunocytochemistry/Immunofluorescence: CACNA2D2 Antibody [NBP2-30044] - Fluorescent image of U251 cell stained with CACNA2D2 Antibody (Center)(NBP2-30044).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CACNA2D2 primary antibody (1:25, 1 h at 37). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37).CACNA2D2 immunoreactivity is localized to Cytoplasm significantly.
![Immunohistochemistry-Paraffin: CACNA2D2 Antibody [NBP2-30044] - Immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of (Center) for immunohistochemistry. Clinical relevance has not been evaluated.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/4117_CACNA2D2-Antibody-Immunohistochemistry-Paraffin-NBP2-30044-img0003.jpg)
Immunohistochemistry-Paraffin: CACNA2D2 Antibody [NBP2-30044] - Immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
![Western Blot: CACNA2D2 Antibody [NBP2-30044] - Western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the CACNA2D2 antibody detected the CACNA2D2 protein (arrow).](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/4115_CACNA2D2-Antibody-Western-Blot-NBP2-30044-img0002.jpg)
Western Blot: CACNA2D2 Antibody [NBP2-30044] - Western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the CACNA2D2 antibody detected the CACNA2D2 protein (arrow).