Western Blot: ATAD1 Antibody (S125-10) [NBP2-12921] - analysis of Rat brain membrane lysate showing detection of ATAD1 ATPase protein using Mouse Anti-ATAD1 ATPase Monoclonal Antibody, Clone S125-10. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-ATAD1 ATPase Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/Immunofluorescence: ATAD1 Antibody (S125-10) [NBP2-12921] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-ATAD1 ATPase Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic and accumulates around nucleus.
Immunohistochemistry-Paraffin: ATAD1 Antibody (S125-10) [NBP2-12921] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-ATAD1 ATPase Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear staining in all basal cells.