Western Blot: Otx1 Antibody (1F2) [H00005013-M01] - Analysis of OTX1 expression in transfected 293T cell line by OTX1 monoclonal antibody (M01), clone 1F2.Lane 1: OTX1 transfected lysate(37.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: Otx1 Antibody (1F2) [H00005013-M01] - Analysis of monoclonal antibody to OTX1 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Otx1 Antibody (1F2) [H00005013-M01] - Analysis of OTX1 over-expressed 293 cell line, cotransfected with OTX1 Validated Chimera RNAi ( Cat # H00005013-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with OTX1 monoclonal antibody (M01), clone 1F2 (Cat # H00005013-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: Otx1 Antibody (1F2) [H00005013-M01] - Detection limit for recombinant GST tagged OTX1 is approximately 0.03ng/ml as a capture antibody.