Western Blot: Kaiso Antibody (2B2) [H00010009-M01] - Analysis of ZBTB33 expression in transfected 293T cell line by ZBTB33 monoclonal antibody (M01), clone 2B2.Lane 1: ZBTB33 transfected lysate(74.6 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: Kaiso Antibody (2B2) [H00010009-M01] - Analysis of monoclonal antibody to ZBTB33 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Kaiso Antibody (2B2) [H00010009-M01] - Analysis of ZBTB33 over-expressed 293 cell line, cotransfected with ZBTB33 Validated Chimera RNAi ( Cat # H00010009-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZBTB33 monoclonal antibody (M01), clone 2B2 (Cat # H00010009-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: Kaiso Antibody (2B2) [H00010009-M01] - Detection limit for recombinant GST tagged ZBTB33 is approximately 0.1ng/ml as a capture antibody.