Western Blot: PGRMC1 Antibody [NBP2-36525] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN and RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-PGRMC1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposuretime of 30 seconds.
Immunohistochemistry: PGRMC1 Antibody [NBP2-36525] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PGRMC1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunoprecipitation: PGRMC1 Antibody [NBP2-36525] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using RIPA lysis buffer. For blotting immunoprecipitated PGRMC1,it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.