Western Blot: ZCRB1 Antibody [NBP2-37757] - Detection of Human ZCRB1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZCRB1 antibody used for IP at 6 ug per reaction. ZCRB1 was also immunoprecipitated by rabbit anti-ZCRB1 antibody. For blotting immunoprecipitated ZCRB1, NBP2-37757 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: ZCRB1 Antibody [NBP2-37757] - Detection of Human and Mouse ZCRB1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZCRB1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.