Immunocytochemistry/Immunofluorescence: Septin-9 Antibody [NBP1-28711] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Septin 9 used at a dilution of 1:1,000 (1ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100.
Immunoprecipitation: Septin-9 Antibody [NBP1-28711] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Septin 9 antibody NBP1-28711 used for IP at 3 ug/mg lysate. Septin 9 was also immunoprecipitated by rabbit anti-Septin 9 antibody NBP1-28764, which recognizes an upstream epitope. For blotting immunoprecipitated Septin 9, NBP1-28764 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.