Western Blot: PRPF38A Antibody [NBP2-37697] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PRPF38A antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: PRPF38A Antibody [NBP2-37697] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PRPF38A antibody used for IP at 6 ug per reaction. PRPF38A was also immunoprecipitated by rabbit anti-PRPF38A antibody. For blotting immunoprecipitated PRPF38A, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.