Western Blot: DGCR14 Antibody [NBP2-36530] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat,mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DGCR14 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: DGCR14 Antibody [NBP2-36530] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated DGCR14, it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.